Identification of candidate antimicrobial peptides derived from abalone hemocyanin
2014
Zhuang, Jun | Coates, Christopher | Zhu, Hongtao | Zhu, Ping | Wu, Zujian | Xie, Lianhui | Fujian Agriculture and Forestry University | Biological and Environmental Sciences | Chinese Academy of Sciences | Chinese Academy of Sciences | Fujian Agriculture and Forestry University | Fujian Agriculture and Forestry University
Haemocyanins present in invertebrate hemolymph are multifunctional proteins, responsible for oxygen transport and contributing to innate immunity through phenoloxidase-like activity. In arthropods, haemocyanin has been identified as a source of broad-spectrum antimicrobial peptides during infection. Conversely, no haemocyanin-derived antimicrobial peptides have been reported for molluscs. The present study describes a putative antimicrobial region, termed haliotisin, located within the linking sequence between the α-helical domain and β-sheet domain of abalone (Haliotis tuberculata) haemocyanin functional unit E. A series of synthetic peptides based on overlapping fragments of the haliotisin region were tested for their bactericidal potential. Incubating Gram-positive and Gram-negative bacteria in the presence of certain haliotisin peptides, notably peptides 3-4-5 (DTFDYKKFGYRYDSLELEGRSISRIDELIQQRQEKDRTFAGFLLKGFGTSAS) led to reductions in microbial growth. Furthermore, transmission electron micrographs of haliotisin-treated bacteria revealed damages to the microbial cell wall. Data discussed here provides the first evidence to suggest that molluscan haemocyanin may act as a source of anti-infective peptides.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل University of Stirling