Opportunities and limitations of molecular methods for quantifying microbial compliance parameters in EU bathing waters
2013
Oliver, David | Van Niekerk, Melanie | Kay, David | Heathwaite, A Louise | Porter, Jonathan | Fleming, Lora E | Kinzelman, Julie | Connolly, Elaine | Cummins, Andy | McPhail, Calum | Rahman, Amanna | Thairs, Ted | de Roda Husman, Ana Maria | Hanley, Nicholas | Dunhill, Ian | Quilliam, Richard | Natural Environment Research Council | Biological and Environmental Sciences | Biological and Environmental Sciences | Aberystwyth University | Lancaster University | Environment Agency | University of Exeter | City of Racine - Health Department | Department for Environment Food & Rural Affairs (DEFRA) | Surfers Against Sewage | Scottish Environment Protection Agency (SEPA) | Environment Agency | UK Water Industry Research | National Institute for Public Health and the Environment | Economics | Environment Agency | Biological and Environmental Sciences | 0000-0002-6200-562X | 0000-0001-7020-4410
The debate over the suitability of molecular biological methods for the enumeration of regulatory microbial parameters (e.g. Faecal Indicator Organisms [FIOs]) in bathing waters versus the use of traditional culture-based methods is of current interest to regulators and the science community. Culture-based methods require a 24-48 hour turn-around time from receipt at the laboratory to reporting, whilst quantitative molecular tools provide a more rapid assay (approximately 2-3 h). Traditional culturing methods are therefore often viewed as slow and ‘out-dated', although they still deliver an internationally ‘accepted' evidence-base. In contrast, molecular tools have the potential for rapid analysis and their operational utility and associated limitations and uncertainties should be assessed in light of their use for regulatory monitoring. Here we report on the recommendations from a series of international workshops, chaired by a UK Working Group (WG) comprised of scientists, regulators, policy makers and other stakeholders, which explored and interrogated both molecular (principally quantitative polymerase chain reaction [qPCR]) and culture-based tools for FIO monitoring under the European Bathing Water Directive. Through detailed analysis of policy implications, regulatory barriers, stakeholder engagement, and the needs of the end-user, the WG identified a series of key concerns that require critical appraisal before a potential shift from culture-based approaches to the employment of molecular biological methods for bathing water regulation could be justified.
اظهر المزيد [+] اقل [-]Additional co-authors: Lidija Globevnik, Valerie J. Harwood, Chris J. Hodgson, David N. Lees, Gordon L. Nichols, Andreas Nocker, Ciska Schets
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل University of Stirling