Application of microsatellite DNA method in determining genetic diversity of farmed broodstocks of Penaeus vannamei (Boone, 1931) in hatchery centers of Bushehr province
2022
Mohammad Ali Nazari | Laleh Mosavi Dehmordi | Mohammad Amini Charmhini | Mohammad Khalil Pazir
The aim of this study was to detect different populations of Pacific White Shrimp (Penaeus vannamei: Boone, 1931) in farmed broodstocks and to determine their genetics indeces in hatchery centers of Bushehr province. First, the origin of the farmed broodstocks was determined based on the historical information of the different stocks. Then, DNA was extracted from muscle tissue of 30 broodstocks from 11 different stocks using a commercial kit. The repeated sequences were amplified using 10 specific primers by PCR in Iranian Shrimp Center. The results showed that the number of alleles was in range of 4.5-5.5 in studied stocks. The most allele frequency was observed in the farmed broadstocks of the third stock in H2 hatchery center, 5.2±0/359. Also, the average of observed heterozygosity in the farmed broadstocks of the second stock in H5 hatchery center obtained 0.669±0.152 that was more than other stocks among all centers. The results of the present study showed that genetics indeces of of third stock in H2 hatchery center were increased compared to other stocks in Bucher province. The inbreeding coefficients of the first stocks in H1 (0.595±0.105) and H3 (0.547±0.145) hatchery centers were significantly higher than other stocks. The inbreeding coefficient increase in farmed broadstocks in these stocks could be related to the small size of founder population and the bias selection programs. However, according to the information from other hatchery centers in Bushehr province and other countries, this amount is acceptable. According to the obtained results, it can be concluded that to increase the genetic indicators in the centers, breeders with higher diversity should be used.
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