Systematic Genomic Surveillance of SARS-CoV-2 Virus on Illumina Sequencing Platforms in the Slovak Republic—One Year Experience
2022
Diana Rusňáková | Tatiana Sedláčková | Peter Radvák | Miroslav Böhmer | Pavol Mišenko | Jaroslav Budiš | Silvia Bokorová | Nikola Lipková | Michaela Forgáčová-Jakúbková | Tomáš Sládeček | Jozef Sitarčík | Werner Krampl | Michaela Gažiová | Anna Kaliňáková | Edita Staroňová | Elena Tichá | Terézia Vrábľová | Lucia Ševčíková | Barbora Kotvasová | Lucia Maďarová | Soňa Feiková | Kristína Beňová | Lenka Reizigová | Zuzana Onderková | Dorota Ondrušková | Dušan Loderer | Mária Škereňová | Zuzana Danková | Katarína Janíková | Erika Halašová | Elena Nováková | Ján Turňa | Tomáš Szemes
To explore a genomic pool of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during the pandemic, the Ministry of Health of the Slovak Republic formed a genomics surveillance workgroup, and the Public Health Authority of the Slovak Republic launched a systematic national epidemiological surveillance using whole-genome sequencing (WGS). Six out of seven genomic centers implementing Illumina sequencing technology were involved in the national SARS-CoV-2 virus sequencing program. Here we analyze a total of 33,024 SARS-CoV-2 isolates collected from the Slovak population from 1 March 2021, to 31 March 2022, that were sequenced and analyzed in a consistent manner. Overall, 28,005 out of 30,793 successfully sequenced samples met the criteria to be deposited in the global GISAID database. During this period, we identified four variants of concern (VOC)—Alpha (B.1.1.7), Beta (B.1.351), Delta (B.1.617.2) and Omicron (B.1.1.529). In detail, we observed 165 lineages in our dataset, with dominating Alpha, Delta and Omicron in three major consecutive incidence waves. This study aims to describe the results of a routine but high-level SARS-CoV-2 genomic surveillance program. Our study of SARS-CoV-2 genomes in collaboration with the Public Health Authority of the Slovak Republic also helped to inform the public about the epidemiological situation during the pandemic.
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