Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology
2015
Pablo F Caligiore-Gei | Jorge G Valdez
The use of a Neubauer chamber is a broadly employed method when cell suspensions need to be quantified. However, this technique may take a long time and needs trained personnel. Spectrophotometry has proved to be a rapid, simple and accurate method to estimate the concentration of spore suspensions of isolates of the genus Fusarium. In this work we present a linear formula to relate absorbance measurements at 530 nm with the number of microconidia/ml in a suspension.
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