OBTAINING OF PROTEIC BIOMASS BY CULTIVATION OF LACTIC ACID BACTERIA ON GRAPE MARC DIFFUSION SOLUTION

Marian BUTU; Steliana RODINO; Petruta PETRACHE; Marius Mihai MICU; Valentina TUDOR

OBTAINING OF PROTEIC BIOMASS BY CULTIVATION OF LACTIC ACID BACTERIA ON GRAPE MARC DIFFUSION SOLUTION

2013

Marian BUTU | Steliana RODINO | Petruta PETRACHE | Marius Mihai MICU | Valentina TUDOR

In this article are presented the researches made in order to obtain protein biomass with the aid of lactic bacteria grown on an economically medium, achieved by using secondary products from the winery: marc and wine yeast. Therefore, there were cultivated two strains of Lactobacillus sp. on five different growth medium. The protein biosynthesis and evolution of lactic fermentation were monitored by determining the optical density (OD) of the culture at a wavelength λ = 600 nm and by counting the colony forming units (CFU) by serial dilutions and seeding on plates and by determination of lactic acid obtained. The results showed that the fermentation medium represented by diffusion solution of the marc, enriched with peptone is economically profitable compared to other culture media containing peptone, yeast extract, glucose, minerals, amino acids and vitamins presented in the literature.

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