Analysis of Soluble Sugar Components and Contents in Fruits from Different Wampee Germplasm Resources

【Objective】Fruit quality indexes including single fruit weight, fruit shape index, total soluble solids (TSS) content, total soluble sugars content, total acids (TA) content and sugar components content of 64 wampee germplasm resources were investigated in order to provide theoretical basis for the breeding of high-quality wampee cultivars.【Method】The anthrone colorimetry and acid-base titration method were used to measure the contents of TSS and TA in wampee fruits, respectively. The sugar component and content were analyzed by High Performance Liquid Chromatography (HPLC) simultaneously. Taking fruit shape index and TSS into account together, the principal component analysis was conducted to screen out high-quality wampee germplasms.【Result】The single fruit weight of these cultivars (lines) ranged from 3.82 to 13.17 g, and the fruit shape index was 0.94-1.57, with an average value of 1.1. The TSS ratio was 10.96%-24.55%, with an average value of 18.08%. The content of total soluble sugar was 8.04%-16.96%, with an average value of 11.88%. The content of TA ranged from 0.11% to 1.95%, with an average value of 1.01%. The contents of sucrose, glucose and fructose were 18.65-74.85 mg/g, 9.7-32.1 mg/g and 11.15-46.85 mg/g, respectively, while the corresponding average contents were 44.28, 20.00 and 22.56 mg/g. Particularly, the total soluble sugar content of Yingzui wampee (008) was the highest, reaching 16.96%, while Taxia sweet wampee (086) p ossessed the highest fructose ratio (35.1%) and sugar-acid ratio (102.14).【Conclusion】Wampee was a type of sucrose accumulation fruit. 10 germplasms with excellent traits were screened out through principal component analysis comprehensively, of which Zaofeng wampee (010), Congcheng sweet wampee (050) and Jinfeng wampee (067) had already been released by Guangdong Crop Variety Approval Committee and all the selected germplasms could be used as candidate materials for breeding of high-quality cultivars and new choice for structure adjustment of wampee varieties.