PECULIARITIES OF THE FEED ADDITIVE “GERMATSINK” INFLUENCE ON MAICRONUTRIENTS AND VEGETATIVE STATUS OF THE PRODUCTIVE COWS

The paper presents the results of the studies of the influence of feed additive “Germatsink” on the content of zinc, copper, iron, magnesium, lithium in the blood cells and serum of cows with different tones of autonomic nervous system. Micronutrients determination was conducted by atomic absorption spectrophotometry in a flame mode. Zinc content in in the blood cells depended on the tone of the autonomic nervous system. Zinc content in blood cells of cows with different autonomic tones increased by 6.8–10.5 % and 9.0–13.3 % from the 10th to the 30th day respectively after feed additive was given. Application of feed additive “Germatsink” showed significant increase of copper content in the blood cells of sympathicotonic cows by 12.9 % (p <0.01) from 10th to 30th day. The conducted studies showed a gradual increase of iron content in the blood cells by the 45th day of the study in normo-, vago- and sympathicotonic cows respectively by 6.8 % (p<0.05), 23.1 % (p<0.01) and 22.8% (p<0.001). Application of feed additive to cows demonstrated an increase in transmembrane potential with iron in the blood of normo-, vago- and sympathicotonic cows by 2.6 %, 15.2 % (p<0.001) and 17.0 % (p<0.01), respectively. Studies showed a gradual increase in the content of manganese in the serum of cows within 45 days, in particular, in normo-, vago- and sympathicotonic cows this index increased, respectively by 12.6 % (p<0.05), 11.3 % and 13.0 %. The results showed a gradual increase of manganese up to 45 day in blood cells of in normo-, vago- and sympathicotonic cows by 6.3 % (p<0.05), 9.3 %, and 10.6 %, respectively. Manganese transmembrane potential index (Mncell/Mnserum) in vago- and sympathicotonic cows was significantly lower before the beginning of the study by 14.2 % (p<0.05) and 16.4 % (p<0.01) respectively. Application of feed additive “Germatsink” showed a gradual increase in the content of lithium in the blood cells of sympathicotonic cows by 12.0 % (p<0.05) up to the 45th day of the study. Application of feed additive to cows did not showed significant changes in the transmembrane potential of lithium. Therefore, the conducted studies showed that application the feed additive “Germatsink” to cows with different vegetative status had a corrective effect on the content of some micronutrients in the blood of animals.