Régénération in vitro de plantes de tomate Lycopersicon esculenteum Mill. adaptées au NaCl
2006
Abir, M. | Hannachi, C. | Zid, E.
In vitro Regeneration of NaCl-adapted Tomato Plants (Lycopersicon esculenteum Mill.). In vitro growth seedlings of tomato (Lycopersicon esculentum Mill. Var. Sabra) were used as source of explants: internode segment and leaf fragment. These explants were cultivated into a MS-based medium containing 0.2 mg.l-1ANA and 2 mg.l-1 BAP, supplemented or not with NaCl at 3 and 6 g.l-1 in order to regenerate salt-tolerant plants. Results show that internode's segment and leaf fragment formed callus both in the control and in the saline media (3 and 6 g.l-1). However NaCl affected calli growth and mineral uptake. The callus growth was stimulated by NaCl at 3 g.I-1, but reduced at 6 g.I-1. Na+ and Cl- content of calli increased in saline media with respect to the control. K+ content decreased slightly with salinity, but Ca2+ content did not change. Leaf callus showed higher K+ and Ca2+ contents both in the control and saline media and were more salt tolerant than internodes callus. Latest ones regenerated shoots only on control medium. Leaf callus adapted to 3 g.l-1 NaCl were able to regenerate shoots, but with high percentage of hyperhydricity. Regenerated plants were rooted and acclimatized successfully and they tolerated 3 g.l-1 NaCl added to irrigation solution, when cultivated on peat under glasshouse.
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