MÉTODOS DE EXTRAÇÃO DE DNA E SELEÇÃO DE PRIMERS DE cpDNA PARA Ficus bonijesulapensis (MORACEAE)
2010
FÁBIO DE ALMEIDA VIEIRA | JOSÉ AUGUSTO DA SILVA SANTANA | RUBENS MANOEL DOS SANTOS | CRISTIANE GOUVÊA FAJARDO | GABRIELA APARECIDA DE OLIVEIRA COELHO | DULCINÉIA DE CARVALHO
Phylogeography has become a powerful approach for elucidating contemporary geographical patterns of evolutionary subdivision within species and species complexes. The aim of this paper was to evaluate the extracted quality of DNA and amplification of noncoding chloroplast DNA for phylogeography studies in Ficus bonijesulapens. The comparative analysis of protocol DNA extraction was based in the CTAB method and protocol from Mogg and Bond. DNA samples were assessed for successful PCR amplification of universal primers for the amplification of noncoding regions of cpDNA. Amplified DNA was separated by electrophoresis, stained with ethidium bromide and photographed under UV light. The protocol Moog e Bond produced the best DNA quality. This method will be used because it produces a high quality DNA in a short time and is less expensive. The primers HA, SG, BF, Q16, F32, FV, DT, CS and JA provided the strongest support for the inferred phylogeography, genetic diversity centers and for successful management in tree-conservation programmes.
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