Selection of <i>Cucumis sativus</i> L. for resistance to fusarium wilt using filtrate of the culture fluid of the fungus <i>Fusarium oxysporum</i> Schlectend
2019
Alexey V. Soldatenko | Anna A. Egorova | Olga V. Baklanova | Alexander N. Hovrin | Lyubov A. Chistyakovа | Oleg A. Razin
Relevance Traditional breeding methods are based on crossing and selection of genotypes among hybrid offspring. In recent decades, along with traditional methods, more and more attention is paid to alternative methods of selection, based on biotechnological manipulations with plants. One of the most important methods of biotechnology is the method of cell selection, which is based on the replacement of the whole plant, as a unit of selection, on its cell. Applying biotechnology techniques from a single plant can produce millions of cells, which increases the chances of finding, eliminating the need for areas for the cultivation of tested plants. As well as accelerating the selection process due to the possibility to carry out the study in the offseason. Methods The studies used the linear material of C. sativus hybrids of All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center and Agroholding "Poisk". Plants were cultivated in laboratory room conditions. As explants used hypocotyl 0.5-1 cm segments isolated from young plants. Results To obtain Cucumis sativus plants with increased resistance to Fusarium by cell selection method, it is recommended to alternate culturing of callus on a non – selective medium containing sucrose in a concentration of 30 g/l, agar – 7 g/l, 0.1 mg/l, NUC – 0.5 mg/l and the filter of the cultural fluid of the fungus in a concentration of 10% within 3 passages.
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