Cytotoxicity and anticoccidial activities of Artemisia sieberi leaf extract: an in vitro study

ABSTRACT For centuries, medicinal plants with abundant supplies of phytochemicals that are physiologically active have been used in traditional medicine. Numerous of these contain anti-inflammatory and antioxidant qualities that help lower the risk of numerous diseases. The illness coccidiosis affects many animals and results in huge monetary losses. Drug-resistant strains of Eimeria spp. have emerged because of drug addiction and usage. Therefore, Artemisia sieberi (Asteraceae family) leaves methanolic extract (ASLE) was assessed for its Phytochemical components, in vitro cytotoxicity, and anticoccidial activity. Using infrared spectroscopy (FT-IR), the components of ASLE were detected. Additionally, different extract concentrations were tested for their anticancer activities when applied to breast cancer cell lines (MCF-7) and lung cancer cell lines (A549). ASLE was prepared and tested in vitro as anticoccidial using the oocyst of Eimeria papillate. Fifteen different functional groups were found to be present in ASLE using (FT-IR). Also, quantitative results showed phenolics and flavonoids of 235.5±2.7 and 47.89 ± 0.3 respectively in ASLE. Moreover, ASLE showed significant cytotoxicity against cancer cells. The LC50 of ASLE was obtained at 98.6± 1.8μg/mL for the A549 and 253.9±4.4μg/mL for the MCF-7 cell lines. At 96 h, significant inhibition of process sporulation for E. papillata oocysts was observed when exposed to ASLE (300mg/mL) and formalin 5%, while amprolium, DettolTM, and phenol showed different levels of inhibition. Our findings demonstrated the presence of anticoccidial in ASLE, which encourages the performance of multiple in vivo investigations to find an effective treatment.