Testicular cell transplantation of neon tetra Paracheirodon innesi into common carp fry

ABSTRACT   Neon tetra Paracheirodon innesi is an ornamental fish that have high export value. However, production is still relatively low due to low fecundity (approximately 180 eggs/female). Technology of testicular cell transplantation of neon tetra as donor to common carp as recipient fish which have high fecundity provides a promising way to overcome problem of neon tetra production. This research was performed to determine the optimum age of common carp fry that is able to receive donor cells and allow high success of transplantation. In this research, the testes of neon tetra fish were dissociated by 0.5% trypsin solution. The testicular cells were labeled with PKH-26 fluorescent dye, and then transplanted into the peritoneal cavity of seven, ten, and 14 days post hatching common carp fry. The results showed that the survival of seven day-old transplanted fry (31.25%) was lower than that of ten day-old (37.75%) and 14 day-old transplanted fry (56.25%). Percentage of fish colonized testicular cells donor at 21 days post-transplantation on seven days old and ten days old fry were similar (80%), while on 14 day-old fry was 60%. Based on the cumulative transplantation success rate (survival and colonization rates), transplantation on 14 days old fry (33.75%) showed higher result compared to transplantation on seven days old fry (25.00%) and ten day-old fry (30.00%). It can be concluded that transplantation of neon tetra testicular cells to common carp fry have been successfully carried out, and the optimum age of common carp fry to transplantation was 14 days after hatching.   Keywords: transplantation, colonization, testicular cells, common carp, neon tetra     ABSTRAK   Ikan neon tetra Paracheirodon innesi merupakan ikan hias yang memiliki nilai ekspor yang tinggi. Namun demikian, tingkat produksinya masih relatif rendah karena fekunditas ikan neon tetra yang sedikit (sekitar 180 telur/induk). Teknologi transplantasi sel testikular ikan neon tetra (ikan donor) ke ikan mas yang memiliki fekunditas telur yang banyak dan diharapkan mampu mengatasi ketersediaan benih ikan neon tetra. Penelitian ini bertujuan untuk menentukan umur optimum benih ikan mas (calon ikan semang) yang mampu menerima sel donor dengan baik dan memiliki keberhasilan kolonisasi yang tinggi. Testis ikan neon tetra didisosiasi menggunakan larutan tripsin 0,5%. Sel testikular diwarnai dengan PKH-26, kemudian ditransplantasikan ke rongga peritoneal benih ikan mas umur tujuh, sepuluh, dan 14 hari setelah menetas. Hasil penelitian menunjukkan bahwa tingkat kelangsungan hidup ikan mas perlakuan transplantasi umur tujuh hari (31,25%) lebih rendah dibandingkan dengan perlakuan transplantasi umur sepuluh hari (37,50%) dan 14 hari (56,25%). Persentase ikan terkolonisasi sel donor pada hari ke-21 pascatransplantasi pada benih umur tujuh dan sepuluh hari adalah sama (80%), sedangkan transplantasi benih umur 14 hari sebesar 60%. Berdasarkan keberhasilan transplantasi secara kumulatif (tingkat kelangsungan hidup dan kolonisasi), transplantasi pada benih umur 14 hari (33,75%) menunjukkan hasil lebih tinggi dibandingkan dengan perlakuan transplantasi pada benih umur tujuh hari (25,00%) dan benih umur sepuluh hari (30,00%). Transplantasi sel testikular ikan neon tetra pada benih ikan mas telah berhasil dilakukan, dan umur optimum benih ikan mas adalah 14 hari setelah menetas.   Kata kunci: transplantasi, kolonisasi, sel testikular, ikan mas, ikan neon tetra