Occurrence of 2-hydroxy fatty acids in animal tissues*
1963
Yasuo Kishimoto | Norman S. Radin
The contents of 2-hydroxy fatty acids in rat spleen, kidney, lung, sciatic nerve, and skin, and in bovine plasma, were determined by a copper precipitation method, and the proportions of the various hydroxy acids were determined by gas-liquid chromatography. It was observed that there are two groups of hydroxy acids, short chain (16h:0 as major component) and long chain (24h:0 as major component). The relative proportions of the two groups varied with the tissue. No 2-hydroxy acids could be found in liver and epididymal fat. The 2-hydroxy acids of brain are not liberated by mild alkali and therefore occur only in amide linkage. The amide-bound fatty acids of brain gangliosides and sphingomyelin account for none, or only a very small fraction, of the total brain hydrosy acids.
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