Disinfection of environmental objects against coccidia oocysts in broiler chickens

The purpose of the research is developing a method for disinfection of environmental objects against coccidia oocysts in broiler chickens.Materials and methods. At the VNIIP – FSC VIEV vivarium, a bioassay test was performed to experimentally infect 60 chickens aged 14 days which were divided into six equivalent groups of 10 birds each and kept isolated in cages. Chickens from the first, second and third groups were administered orally, using a micropipette, 1 ml of an Eimeria oocyst suspension treated with 4, 5 and 6% solutions of the combined eimeriocide agent. Chickens from the fourth group were administered 1 ml of Eimeria oocyst suspension treated with 4% phenol solution (base drug). Chickens from the fifth group received 1 ml of a buffered solution and were used as a noninfected control. Chickens from the sixth group received 1 ml of suspension containing 2000 oocysts/mL and were used as an infected control. The efficacy of disinfection with eimeriocide and the basic drug was determined based on the percentage of decrease in the recovery of Eimeria oocysts after being exposed to drugs as compared to chickens of the infected control. The efficacy of 5% eimeriocide against poultry coccidia oocysts in a production test was determined empirically with the set of oocysts on control sites as compared with the basic drug on a poultry farm in the Moscow Region.Results and discussion. The intense-effectiveness of 4% eimeriocide against coccidia oocysts was 99.31%, and the 5 and 6% combined agent showed 100% efficacy. The basic drug, 4% phenol showed 74.65% intense-effectiveness. The results obtained in the production test of 5% eimeriocide at a dose of 0.5 l per 1 m2 with a 2 hour-exposure indicate its high efficacy for disinfection against coccidia oocysts in broiler chickens. The intense-effectiveness was 97.25% versus 59.03% efficacy of the base drug, phenol.