Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications
2021
Nadide Altincekic | Nadide Altincekic | Sophie Marianne Korn | Sophie Marianne Korn | Nusrat Shahin Qureshi | Nusrat Shahin Qureshi | Marie Dujardin | Martí Ninot-Pedrosa | Rupert Abele | Marie Jose Abi Saad | Caterina Alfano | Fabio C. L. Almeida | Fabio C. L. Almeida | Islam Alshamleh | Islam Alshamleh | Gisele Cardoso de Amorim | Gisele Cardoso de Amorim | Thomas K. Anderson | Cristiane D. Anobom | Cristiane D. Anobom | Chelsea Anorma | Jasleen Kaur Bains | Jasleen Kaur Bains | Adriaan Bax | Martin Blackledge | Julius Blechar | Julius Blechar | Anja Böckmann | Louis Brigandat | Anna Bula | Matthias Bütikofer | Aldo R. Camacho-Zarco | Teresa Carlomagno | Teresa Carlomagno | Icaro Putinhon Caruso | Icaro Putinhon Caruso | Icaro Putinhon Caruso | Betül Ceylan | Betül Ceylan | Apirat Chaikuad | Apirat Chaikuad | Feixia Chu | Laura Cole | Marquise G. Crosby | Vanessa de Jesus | Vanessa de Jesus | Karthikeyan Dhamotharan | Karthikeyan Dhamotharan | Isabella C. Felli | Isabella C. Felli | Jan Ferner | Jan Ferner | Yanick Fleischmann | Marie-Laure Fogeron | Nikolaos K. Fourkiotis | Christin Fuks | Boris Fürtig | Boris Fürtig | Angelo Gallo | Santosh L. Gande | Santosh L. Gande | Juan Atilio Gerez | Dhiman Ghosh | Francisco Gomes-Neto | Francisco Gomes-Neto | Oksana Gorbatyuk | Serafima Guseva | Carolin Hacker | Sabine Häfner | Bing Hao | Bruno Hargittay | Bruno Hargittay | K. Henzler-Wildman | Jeffrey C. Hoch | Katharina F. Hohmann | Katharina F. Hohmann | Marie T. Hutchison | Marie T. Hutchison | Kristaps Jaudzems | Katarina Jović | Janina Kaderli | Gints Kalniņš | Iveta Kaņepe | Robert N. Kirchdoerfer | John Kirkpatrick | John Kirkpatrick | Stefan Knapp | Stefan Knapp | Robin Krishnathas | Robin Krishnathas | Felicitas Kutz | Felicitas Kutz | Susanne zur Lage | Roderick Lambertz | Andras Lang | Douglas Laurents | Lauriane Lecoq | Verena Linhard | Verena Linhard | Frank Löhr | Frank Löhr | Anas Malki | Luiza Mamigonian Bessa | Rachel W. Martin | Rachel W. Martin | Tobias Matzel | Tobias Matzel | Damien Maurin | Seth W. McNutt | Nathane Cunha Mebus-Antunes | Nathane Cunha Mebus-Antunes | Beat H. Meier | Nathalie Meiser | Miguel Mompeán | Elisa Monaca | Roland Montserret | Laura Mariño Perez | Celine Moser | Claudia Muhle-Goll | Thais Cristtina Neves-Martins | Thais Cristtina Neves-Martins | Xiamonin Ni | Xiamonin Ni | Brenna Norton-Baker | Roberta Pierattelli | Roberta Pierattelli | Letizia Pontoriero | Letizia Pontoriero | Yulia Pustovalova | Oliver Ohlenschläger | Julien Orts | Andrea T. Da Poian | Dennis J. Pyper | Dennis J. Pyper | Christian Richter | Christian Richter | Roland Riek | Chad M. Rienstra | Angus Robertson | Anderson S. Pinheiro | Anderson S. Pinheiro | Raffaele Sabbatella | Nicola Salvi | Krishna Saxena | Krishna Saxena | Linda Schulte | Linda Schulte | Marco Schiavina | Marco Schiavina | Harald Schwalbe | Harald Schwalbe | Mara Silber | Marcius da Silva Almeida | Marcius da Silva Almeida | Marc A. Sprague-Piercy | Georgios A. Spyroulias | Sridhar Sreeramulu | Sridhar Sreeramulu | Jan-Niklas Tants | Jan-Niklas Tants | Kaspars Tārs | Felix Torres | Sabrina Töws | Miguel Á. Treviño | Sven Trucks | Aikaterini C. Tsika | Krisztina Varga | Ying Wang | Marco E. Weber | Julia E. Weigand | Christoph Wiedemann | Julia Wirmer-Bartoschek | Julia Wirmer-Bartoschek | Maria Alexandra Wirtz Martin | Maria Alexandra Wirtz Martin | Johannes Zehnder | Martin Hengesbach | Andreas Schlundt | Andreas Schlundt
The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.
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