PECULIARITIES OF DISTRIBUTION AND DYNAMICS OF CELLS OF THE CONNECTIVE TISSUE OF THE OVARIANS OF RATS OFFSPRING AFTER THE INTRODUCTION OF PROGESTERONE DURING PREGNANCY

In obstetric and gynecological practice progesterone is often used in cases of miscarriage. The negative effect of progesterone on testicles of rats has been proven. However, the effects of progesterone introduction during pregnancy on fetal ovaries are not sufficiently studied require further study. Objective. To determine the peculiarities of the distribution and dynamics of ovarian connective tissue cells (fibroblasts, fibrocytes, macrophages, mast cells, lymphocytes) in the offspring of rats receiving progesterone during pregnancy. Object and methods. The ovaries of laboratory rats were investigated on the 9th, 14th, 21th, 30th, 45th, 60th and 90th day of postnatal life. The rats included in a study were divided into 3 groups: I intact, II experimental group of rats, who were injected by 0,33 ml of progesterone oil solution on the 15th to 18th day of pregnancy and III control group of rats which were injected by 0.9% Sodium Chloride solution in equivalent dosage. Serial sections were stained with àlcian blue with a 0,2Ì as a critical concentration of MgCl2 . Results and discussion. In animals of the experimental group, compared to control group, a significant increase in the number of fibrocytes and a significant decrease in the number of fibroblasts up to 90 days of life was observed. The macrophage and lymphocyte level changed wavily during the observation period with a tendency to decrease in experimental animals at the end of the third month of life. The number of mast cells in animals of the experimental group increased from the 30th to the 90th day of life inclusively compared to the control group data. Conclusions. The established changes may reflect a disturbance in the processes of growth and maturation of the follicles, as well as in ovulation processes after the antenatal affection of progesterone on the offspring ovaries.