Development and Characterization of Microsatellite Loci in the Endangered Species <i>Taxus wallichiana</i> (Taxaceae)
2013
Jyoti Prasad Gajurel | Carolina Cornejo | Silke Werth | Krishna Kumar Shrestha | Christoph Scheidegger
Premise of the study: Microsatellite primers were developed in the endangered tree species <i>Taxus wallichiana</i> from Nepal to investigate regional genetic differentiation, local genetic diversity, and gene flow for the conservation of this species under climate- and land-use change scenarios in mountain regions of Nepal. Methods and Results: We developed 10 highly polymorphic microsatellite markers from 454 DNA sequencing. Characterization of the new microsatellite loci was done in 99 individuals collected from three valleys with different climatic regimes. The number of alleles per locus varied from four to 12. Observed heterozygosity of populations, averaged across loci, ranged from 0.30 to 0.59. Conclusions: The new markers provided by this study will substantially increase the resolution for detailed studies in phylogeography, population genetics, and parentage analysis.
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