Overexpression of R2R3-MYB gene leads to accumulation of anthocyanin and enhanced resistance to chilling and oxidative stress
2014
X. Meng | B. Yin | H. -L. Feng | S. Zhang | X. -Q. Liang | Q. -W. Meng
LeAN2 encoding an anthocyanin-associated R2R3-MYB transcription factor was isolated from tomato. The expression of LeAN2 in tomato was induced by low temperature and oxidative stress. Green fluorescent protein was fused to LeAN2 and the complex was expressed transiently in onion epidermal cells. Green fluorescence was observed only in the nucleus. Overexpression of LeAN2 under the control of the CaMV35S promoter in tobacco induced expression of several anthocyanin biosynthetic genes and the content of anthocyanin was markedly higher in transgenic tobacco compared with wild type plants. Transgenic tobaccos conferred tolerance to chilling stress by maintaining a higher chlorophyll content, net photosynthetic rate, and maximal photochemical efficiency of PS II compared to the wild type plants. Furthermore, the transgenic plants showed lower ion leakage, a lower content of reactive oxygen species, and a higher content of non-enzymatic antioxidans under the chilling stress. They also showed an enhanced resistance to the oxidative stress induced by methyl viologen based on a decreased chlorophyll content loss, lower ion leakage, and an enhanced maximal photochemical efficiency of PS II. These results indicate that overexpression of LeAN2 resulted in an increased anthocyanin accumulation and enhanced resistance to the chilling and oxidative stresses in transgenic tobacco.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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