The Cysteine Protease CfAtg4 Interacts with CfAtg8 to Govern the Growth, Autophagy and Pathogenicity of <i>Colletotrichum fructicola</i>
2024
Shufeng Guo | Shengpei Zhang
<i>Camellia oleifera</i> is a native woody oil plant in southern China and is infected with anthracnose wherever it is grown. We previously identified <i>Colletotrichum fructicola</i> as the major causal agent of anthracnose on <i>C. oleifera</i> and found that CfAtg8 regulates the pathogenicity and development of <i>C. fructicola</i>. Here, we revealed that CfAtg4 interacts with CfAtg8, contributing to the formation of autophagosomes. The CfAtg8<sup>1–160</sup> allele, which only contains 1–160 amino acids of the CfAtg8, partially recovered the autophagosome numbers and autophagy defects of the Δ<i>Cfatg4</i> mutant. Consequently, these recoveries resulted in the restoration of the defects of the Δ<i>Cfatg4</i> mutant in growth and responses to different external stresses, albeit to an extent. Importantly, we illustrated the critical roles of CfAtg8<sup>1–160</sup> in appressoria formation, and pathogenicity. Collectively, our findings provide new insights into the importance of the interaction between CfAtg8 and CfAtg4 in the growth, autophagy and pathogenicity of the phytopathogenic fungi.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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