Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana
2018
Valérie Allasia | Benoit Industri | Michel Ponchet | Michaël Quentin | Bruno Favery | Harald Keller
Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its’ inactive, vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in Arabidopsis thaliana. This protocol describes a simplified, optimized procedure to extract and quantify free SA and total hydrolyzable SA in plant tissues using a classical HPLC-based method.
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