Is There Any Possibility to Use Ultrasounds, High-Pressure Homogenization or Pulsed Electric Field in Single Cell Oil Release from Oleaginous Yeast Cells?
2022
Katarzyna Wierzchowska | Aleksandra Musiałek | Bartłomiej Zieniuk | Karina Jasińska | Dorota Nowak | Agata Fabiszewska
Microbial oil (SCO) is lipids accumulated in the cells of oleaginous microorganisms, including yeast, in amounts exceeding 20% of dry mass. These are a valuable source of fatty acids in the human diet. In order to facilitate the extraction of storage lipids from cells, methods of physical and chemical pre-treatment of biomass are used to break the barrier of the cell wall and membrane of these microorganisms to the action of organic solvents, which are used during traditional extraction. The aim of this study was to evaluate the effectiveness of unconventional methods of extracting microbial oil from <i>Yarrowia lipolytica</i> yeast cells. Pulsed electric field (PEF), cell disintegration by ultrasonic waves and high-pressure homogenization (HPH) were used. The use of unconventional methods turned out to be ineffective in the extraction of intracellular lipids of the yeast compared to methods involving organic solvents such as chloroform, methanol and hexane. Nevertheless, the use of a pulsed electric field with a field strength of 200 J/g or high-pressure homogenization (1100 bar) proved to be effective as pre-treatment techniques of <i>Y. lipolytica</i> yeast cells (cell permeabilization) for the high yield extraction of intracellular lipids using the extraction method with organic solvents.
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