Seedling performance, phenotypic traits and growing media for domestication of Lilium polyphyllum

Societal Impact Statement Medicinal plants are a key component of primary health care for much of the world's population. However, overharvesting and indiscriminate collection from the wild threatens medicinal plant populations with extinction. One conservation solution to preserve biodiversity is to domesticate and cultivate threatened medicinal plants, in order to provide a sustainable source close to the local populations who use the species. Here, we present work to cultivate the critically endangered (CR) medicinal herb, Lilium polyphyllum. To reduce unsustainable wild harvesting, we identify different climatic zones, habitats and growing media, which will aid domestication of this endangered species and help local populations to cultivate the plant near its natural habitat. Summary Lilium polyphyllum (Liliaceae) is a perennial, bulbous medicinal herb, used in more than 30 Ayurvedic medicinal formulations as an aphrodisiac, galactagogue and anti‐ageing agent. The species is categorized as critically endangered on the IUCN (International Union for Conservation of Nature) Red List due to habitat degradation and unregulated collection. To alleviate the pressure on wild populations and increase conservation, the IUCN Red List recommends its domestication in suitable habitats. The present study was carried out over three consecutive years to evaluate the performance and survival of the species in two agro‐climatic zones in Garhwal Himalaya, Uttarakhand, India. L. polyphyllum seedlings were raised and transplanted into different growing mediums and treatments (control, manure, litter and polyhouse) in both temperate (Pothivasa, 2200 m a.s.l.) and alpine (Tungnath, 3600 m a.s.l.) regions to identify a suitable site and or habitat for future mass production. Experiments revealed yield and performance in polyhouse beds were greatest in the temperate region. Plant survival was also high in polyhouse beds in both the temperate (81%) and alpine (40%) sites. Phenotypic and growth traits of plants grown from bulbs collected from the wild population of the temperate site performed better in both morphology and in yield than those in the alpine site after one growing season. Cocopeat and sand may be responsible for the improved growth of plants. In conclusion, plant survival rate, growth performance and yield under polyhouse conditions were higher in lower altitudes (temperate regions), which may provide a production centre for seedlings to interested farmers in other regions.