Determination of Contents of the Main Nutritional Components, Functional Components of Akebia trifoliata Pericarp and the Antioxidant Activity of Its Extracts
2022
Mengqin ZHANG | Lu XU | Junbo ZHANG | Genyou OU | Yuan XIANG
In order to explore the nutritional value and active function of Akebia trifoliata pericarp, the main nutritional components contents of protein, fat, total sugar, reducing sugar, 8 mineral elements, total flavonoids, total phenols and total saponins were determined by using Kjeldahl method, acid hydrolysis, phenol sulfuric acid method, 3,5-dinitrosalicylic acid method, ICP-AES method, NaNO2-AlCl3 method, Folin-ciocalten and vanillin-perchloric acid. The antioxidant capacity of pericarp extracts was also evaluated. The results showed that the contents of protein (8.16%±0.11%), reducing sugar (19.31%±0.21%) and total sugar (32.61%±0.18%) in the peel of Akebia trifoliata were high, and the pectin content determined by gravimetric method was (20.08%±0.20%). Among the eight nutrient elements measured, K content was the richest (14061.17 μg/g DW), followed by Ca (4959.28 μg/g DW). The orders of the contents of eight mineral elements were K>Ca>Mg>Fe>Mn>Na>Cu>Zn. The contents of total flavonoids, polyphenolics and saponins were (20.58±0.12), (31.53±0.18) and (45.20±12.04) mg/g DW, respectively. The scavenging capabilities of extraction on the free radicals of DPPH and ABTS+ were also investigated. When the scavenging rate reached to 50%, the IC50 values on DPPH and ABTS+ free radicals scavenging were 0.049, 0.052 mg/mL, respectively. The scavenging ability of extracts in Akebia trifoliata pericarp was dose-dependent. The contents of total flavonoids, total polyphenols and total saponins had strong and positive correlations with antioxidant activity (P<0.01). In summary, Akebia trifoliata pericarp should be new resources with high nutritional values and strong antioxidant activities worth for in-depth development.
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