Molecular characterization of sugarcane varieties cultivated in Tabasco state, Mexico
2011
Vianey González-Jiménez | Apolonio Valdez Balero | Fernando Carlos Gómez Merino | Hilda Victoria Silva Rojas | Julián Pérez Flores | Carlos Fredy Ortiz García
To characterize varieties in recent years have used different types of morphological, biochemical and molecular methods. The aim of this study was to characterize molecular genetic variability in varieties of sugarcane (Saccharum spp.) grown in the state of Tabasco, Mexico, by AFLP. It was used 12 varieties of sugarcane. Young leaves of plants growing in the field were selected for experiment. After making 12 combinations of primers, the results indicated that the combination E-ACC/M-CTA produced 72 to 1353 bp polymorphic fragments. The dendrogram revealed two distinct groups of Saccharum spp. and a variety that differed from both. Group I comprised the varieties C 87-51, 96-40 ATM, B 4362, 69-290 Mex, Mex Mex 91-130 and 57-1285, which formed a cluster and showed a similarity of 0.77%. Group II included the varieties RD 75-11, Mex 79-431, SP 70-1284, 72-2086 and CP Mex 59-32 which formed another cluster with 0.70%. The variety Mex 68-P-23 differed from the other 11 varieties. Keywords: DNA, AFLP, molecular markers
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