Histopathological and morphological alterations caused by Plasmodiophora brassicae in Brassica oleracea L.

<p class="MsoNormal" style="text-align: justify; line-height: normal; margin: 0cm 0cm 0pt; mso-layout-grid-align: none;"><em><span style="font-family: "MinionPro-It","serif"; font-size: 9.5pt; mso-bidi-font-family: MinionPro-It; mso-ansi-language: EN-US;" lang="EN-US">Plasmodiophora brassicae </span></em><span style="font-family: "MinionPro-Regular","serif"; font-size: 9.5pt; mso-bidi-font-family: MinionPro-Regular; mso-ansi-language: EN-US;" lang="EN-US">is a plant pathogen of the Brassicaceae family, which presents a remarkable ability to survive in soil and high capacity of infection, significantly reducing crop yields. The present histopathologycal study conducted with the aim of contributing to knowledge of the infection cycle of the pathogen, showed the presence of multinucleated plasmodia at cortex and periderm cells level in infected cabbage roots, as well as thickening and disruption of cell wall. As a result of this disarray was observed in diseased tissues, mainly in the cortex, compared with healthy tissues in uninfected plants. The inoculation cabbage seedlings with dormant spores of </span><em><span style="font-family: "MinionPro-It","serif"; font-size: 9.5pt; mso-bidi-font-family: MinionPro-It; mso-ansi-language: EN-US;" lang="EN-US">P.</span></em><span style="font-family: "MinionPro-Regular","serif"; font-size: 9.5pt; mso-bidi-font-family: MinionPro-Regular; mso-ansi-language: EN-US;" lang="EN-US"> </span><em><span style="font-family: "MinionPro-It","serif"; font-size: 9.5pt; mso-bidi-font-family: MinionPro-It; mso-ansi-language: EN-US;" lang="EN-US">brassicae </span></em><span style="font-family: "MinionPro-Regular","serif"; font-size: 9.5pt; mso-bidi-font-family: MinionPro-Regular; mso-ansi-language: EN-US;" lang="EN-US">at concentrations of 1 ∙ 10</span><span style="font-family: "MinionPro-Regular","serif"; font-size: 5.5pt; mso-bidi-font-family: MinionPro-Regular; mso-ansi-language: EN-US;" lang="EN-US">7 </span><span style="font-family: "MinionPro-Regular","serif"; font-size: 9.5pt; mso-bidi-font-family: MinionPro-Regular; mso-ansi-language: EN-US;" lang="EN-US">and 1 ∙ 10</span><span style="font-family: "MinionPro-Regular","serif"; font-size: 5.5pt; mso-bidi-font-family: MinionPro-Regular; mso-ansi-language: EN-US;" lang="EN-US">8 </span><span style="font-family: "MinionPro-Regular","serif"; font-size: 9.5pt; mso-bidi-font-family: MinionPro-Regular; mso-ansi-language: EN-US;" lang="EN-US">spores mL</span><span style="font-family: "MinionPro-Regular","serif"; font-size: 5.5pt; mso-bidi-font-family: MinionPro-Regular; mso-ansi-language: EN-US;" lang="EN-US">-1 </span><span style="font-family: "MinionPro-Regular","serif"; font-size: 9.5pt; mso-bidi-font-family: MinionPro-Regular; mso-ansi-language: EN-US;" lang="EN-US">by immersion and spray, induced a higher growth and less growth longitudinal lateral roots compared with uninoculated plants as well as presence of young plasmodia at 28 days after inoculation.</span></p>