The Duck <i>RXRA</i> Gene Promotes Adipogenesis and Correlates with Feed Efficiency
2023
Ziyi Pan | Xuewen Li | Dongsheng Wu | Xingyong Chen | Cheng Zhang | Sihua Jin | Zhaoyu Geng
Background: The accumulation of fat in ducks is the main cause of low feed efficiency and metabolic diseases in ducks. Retinoic acid X receptor alpha (<i>RXRA</i>) is a member of the nuclear receptor superfamily involved in lipid, glucose, energy, and hormone metabolism. The effect of the <i>RXRA</i> gene on lipid metabolism in duck preadipocytes (DPACs) and the relationship between SNPs and the feed efficiency traits of ducks are unclear. Methods: qRT-PCR and Western blotting analyses were used to detect changes in mRNA and protein in cells. Intracellular triglycerides (TGs) were detected using an ELISA kit. A general linear model analysis was used to determine the association between <i>RXRA</i> SNPs and feed efficiency. Results: The duck <i>RXRA</i> gene was highly expressed on the fourth day of DPAC differentiation. The <i>RXRA</i> gene increased the content of fat and TG in DPACs and promoted the expression of cell differentiation genes; g.5,952,667 correlated with average daily feed intake (ADFI), residual feed intake (RFI), and feed conversion ratio (FCR). Conclusions: Duck <i>RXRA</i> can accelerate fat accumulation, and the polymorphism of the <i>RXRA</i> gene is closely related to feed efficiency, which provides basic data for breeding high feed efficiency ducks.
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