The Limiting Sequence and Appropriate Amino Acid Ratio of Lysine, Methionine, and Threonine for Seven- to Nine-Month-Old Holstein Heifers Fed Corn–Soybean M-Based Diet

An &#8220;Amino acid (AA) partial deletion method&#8221; was used in this experiment to study the limiting sequences and appropriate ratio of lysine (Lys), methionine (Met), and threonine (Thr) in the diets of 7- to 9-month-old Holstein heifers. The experiment was conducted for three months with 72 Holstein heifers (age = 22 &#177; 0.5 weeks old; BW = 200 &#177; 9.0 kg; mean &#177; standard deviation). Following an initial two weeks adaptation period, heifers were allocated to one of four treatments: a theoretically balanced amino acid diet (positive control [PC]; 1.00% Lys, 0.33% Met, and 0.72% Thr), a 30% Lys deleted diet (partially deleted Lys [PD&#8722;Lys]; 0.66% Lys, 0.33% Met, and 0.72% Thr), a 30% Met deleted diet (partially deleted Met, [PD&#8722;Met]; 1.00% Lys, 0.22% Met, and 0.72% Thr), and a 30% Thr deleted diet (partially deleted Thr [PD&#8722;Thr]; 1.00% Lys, 0.33% Met, and 0.45% Thr). Experimental animals were fed a corn&#8722;soybean meal-based concentrate and alfalfa hay. In addition, the animals were provided with supplemental Lys, Met, and Thr (ruminal bypass). The results found no differences in the growth performance and nitrogen retention between PD&#8722;Thr treatment and PC treatment (<i>p</i> &gt; 0.05). The average daily gain (<i>p</i> = 0.0013) and feed conversion efficiency (<i>p</i> = 0.0057) of eight- to ninr-month-old heifers were lower in both PD&#8722;Lys and PD&#8722;Met treatment than those in PC treatment. According to growth performance, Lys was the first limiting AA, followed by Met and Thr. Moreover, nine-month-old Holstein heifers in PD&#8722;Lys treatment and PD&#8722;Met treatment had higher levels of serum urea nitrogen (<i>p</i> = 0.0021), urea nitrogen (<i>p</i> = 0.0011) and total excreted N (<i>p</i> = 0.0324) than those in PC treatment, which showed that nitrogen retention significantly decreased (<i>p</i> = 0.0048) as dietary Lys and Met levels decreased. The limiting sequence based on nitrogen retention was the same as that based on growth performance. The appropriate ratio of Lys, Met, and Thr in the diet based on nitrogen retention was 100:32:57. In summary, the limiting sequence and appropriate amino acid ratio of Lys, Met, and Thr for seven- to nine-month-old Holstein heifers fed a corn&#8722;soybean meal-based diet were Lys &gt; Met &gt; Thr and 100:32:57, respectively.