Effects of the herbal preparation AyuFertin, used for anestrus overcome, on fatty acids composition of milk in Bulgarian Murrah buffaloes

The herbal protocols, used instead of hormones in buffalo breeding for reproduction optimisation, do not provide information of their effect on the milk quality of treated animals. This research analysed whether the herbal preparation AyuFertin (Indian Herbs Specialities Pvt. Ltd, Saharanpur), applied in postpartum period for recovering the ovary cycling, had a side effect on the milk composition. The experiment was conducted with buffaloes of the Bulgarian Murrah breed at average age of 50 months during May-June 2019, divided into control (n=6) and experimental (n=7) groups. The experimental buffaloes received AyuFertin orally for three consecutive days at a dose of 3 g/100 kg live weight since the 21st postpartum day. The animals without signs of estrus were treated again after 10 days. The milk samples were collected at test-days, before and after treatments. The fatty acid composition of the milk and Ayufertin was evaluated by gas chromatography-mass spectrometry analysis. Quanti-fication of the identified fatty acids in milk was made by the area normalisation method. In general, the fatty acids composition did not differ after the treatment with AyuFertin. Deviation in the amounts of some fatty acids was established. The content of medium-chain fatty acids such as lauric and carpic was decreased, while the amount of long-chain acids such as 11-hexadecanoic and stearic was in-creased in the experimental group. The main active components of AyuFertin  trienoic acids were not transferred into the milk of treated animals. In conclusion, due to the ability of AyuFertin to affect the fatty acids amount in the milk of treated buffaloes, farmers should strictly follow the manufac-turer’s recommendation for the dose and duration of AyuFertin treatment to avoid negative effects on milk quality.