Polymorphisms in the bovine tumour necrosis factor receptor type two gene (TNF-RII) and cell subpopulations naturally infected with bovine leukaemia virus

Stachura Alicja; Bojarojć-Nosowicz Barbara; Kaczmarczyk Dariusz; Kaczmarczyk Ewa

Polymorphisms in the bovine tumour necrosis factor receptor type two gene (TNF-RII) and cell subpopulations naturally infected with bovine leukaemia virus

2019

Stachura Alicja | Bojarojć-Nosowicz Barbara | Kaczmarczyk Dariusz | Kaczmarczyk Ewa

Introduction: Numerous mutations in the bovine tumour necrosis factor receptor type two (TNF-RII) gene have been identified, but their biological consequences remain poorly understood. The aim of this study was to determine whether polymorphism in the analysed loci of the bovine TNF-RII gene is linked with the size of cell subpopulations naturally infected with bovine leukaemia virus (BLV) which serve important immune functions in the host. Material and Methods: Samples originated from 78 cows. Polymorphisms in the studied gene were determined by PCR-RFLP and DNA sequencing by capillary electrophoresis. BLV infection was diagnosed by the immunofluorescence (IMF) technique and nested PCR. Cell subpopulations were immunophenotyped with IMF. Results: Similar and non-significant differences in the average percentages of TNFα+, IgM+TNFα+, and CD11b+TNFα+ cells infected with BLV were noted in individuals with various genotypes in the polymorphic sites g.-1646T > G and g.16534T > C of the TNF-RII gene, and significant differences in the percentages of these subpopulations were observed between selected microsatellite genotypes (g.16512CA(n)). Conclusion: STR polymorphism and the number of CA dinucleotide repeats in intron 1 of the TNF-RII gene influence the frequency of TNF+, CD11b+TNF+, and IgM+TNF+ subpopulations naturally infected with BLV. Polymorphism in the gene’s other two sites do not affect the size of these cell subpopulations.

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