The efficient production of dairy cattle and Wakasagyu (Japanese Black) using embryo transfer (ET)
2018
Matsumoto, S. (Fukui Prefectural Livestock Experiment Station (Japan)) | Kobayashi, T. | Sasaki, K.
We transfered female embryo to the opposite uterine horn of corpus luteum following to artificial insemination using sexed sperm to daily cattle (ET after AI). The availability of lnterferon-stimulated gene protein 15 kDa (ISG15) for pregnancy diagnosis for twins was also examined. For the results, the fertility of ET after AI was 36.0% (9/25 cattle) and, was higher than the fetility of AI using sexed sperm (25.9%, 7/27 cattle) and ET (30.4%, 95/313 cattle). The total cholesterol of fertilized cattle was higher than unfertilized; therefore it could be said that the shortage of calorie caused lower the fertility rate. The expression level of ISG15 indicated 50% of twin predictive rate (twin predicted / twin pregnancy: 1/2 cattle), and it might be that this low twin predictive rate showed the determination of ISG15 could not be used for prediction of twin.
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