Temperature- and salinity-decoupled overproduction of hydroxyectoine by chromohalobacter salexigens
2012
Nieto Gutiérrez, Joaquín José | Rodríguez Moya, Javier | Argandoña Bertrán, Montserrat | Iglesias Guerra, Fernando | Vargas Macías, Carmen | Universidad de Sevilla. Departamento de Microbiología y Parasitología | Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica
Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hy- droxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines.
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