Stock identification and spawning depth of oval squid Sepioteuthis spp. in Tanega-shima Island inferred by DNA markers
2015
Tomano, S. (Hiroshima University, Higashi-Hiroshima, Hiroshima (Japan). Graduate School of Biosphere Science) | Ueta, Y. | Kasaoka, N. | Umino, T.
Stock identification of oval squid Sepioteuthis spp. in the coastal waters of Tanega-shima Island were examined using ten polymorphic microsatellite loci and partial nucleotide sequences of cytochrome oxidase subunit I (COI) in the mtDNA. Also, we suggested spawning depth through eggs identification collected from artificial reefs from 20-50 m in depth. Adult specimens collected from Tanega-shima Island, identified as Akaika Sepioteuthis sp. 1 based on phenotypic characters and Tokushima waters (referred as Shiroika Sepioteuthis sp. 2) were genetically differentiated in both microsatellite allele frequencies and mtDNA sequences, which supported presence of reproductive isolation inferred by isozyme analysis. Eggs and embryos at three artificial reefs from 40-50 m in depth were identified as Sepioteuthis sp. 1, except for 20 m. Preset results indicate that Sepioteuthis sp. 1 living in Tanega-shima Island utilize 40-50 m in depth as a spawning zone. The partial COI sequences used in the present study allowed inferring phylogenetic relationship among Sepioteuthis spp. generated from Indo-Pacific region and detecting co-existence of Sepioteuthis sp. 1 and sp. 2 in Vietnam and Indonesia.
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