Regulation of myometrial contraction by ATP-sensitive potassium (Ksub(ATP)) channel via activation of SUR2B and Kir 6.2 in mouse
2016
Hong, S.H. (Chungbuk National University (CBNU), Cheongju, Chungbuk (Korea). Department of Obstetrics and Gynecology) | Kyeong, K.S. | Kim, C.H. | Kim, Y.C. | Choi, W. | Yoo, R.Y. | Kim, H.S. | Park, Y.J. | Ji, I.W. | Jeong, E.H. | Kim, H.S. | Xu, W.X. | Lee, S.J.
ATP-sensitive potassium (Ksub(ATP)) channels are well characterized in cardiac, pancreatic and many other muscle cells. In the present study, functional expression of the Ksub(ATP) channel was examined in non-pregnant murine longitudinal myometrium. Isometric contraction measurements and Western blot were used. Ksub(ATP) channel openers (KCOs), such as pinacidil, cromakalim, diazoxide and nicorandil, inhibited spontaneous myometrial contractions in a reversible and glibenclamide-sensitive manner. KCOs inhibited oxytocin (OXT)- and prostaglandin Fsub(2alpha) (PGFsub(2alpha))-induced phasic contractions in a glibenclamide-sensitive manner. SUR2B and Kir6.2 were detected by Western blot, whereas SUR1, SUR2A and Kir6.1 were not. These results show that pinacidl, cromakalim, diazoxide and nicorandil-sensitive Ksub(ATP) channels exist in murine myometrium, which are composed of SUR2B and Kir6.2. Based on the modulatory effects of the Ksub(ATP) channel on spontaneous contraction, OXT- and PGFsub(2alpha)-induced contractions, Ksub(ATP) channels seem to play an essential role in murine myometrial motility via activation of SUR2B and Kir6.2.
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