Hammer blot-mediated RNA extraction: An inexpensive, labor-saving method to extract RNA for plant virus detection
2016
Akhter, M.S. (Ehime University, Matsuyama, Ehime (Japan). The United Graduate School of Agricultural Sciences) | Yaeno, T. | Yamaoka, N. | Nishiguchi, M. | Kobayashi, K.
Here we report a novel method to extract nucleic acids for virus detection. Plant tissue in a filter paper sandwich was hit with a hammer, and the crude sap was adsorbed by the filter paper, as in hammer blotting for tissue-print immunodetection of plant viruses. Nucleic acids were extracted from the paper with a guanidine-containing buffer and purified through standard extraction and precipitation. Both viral and cellular RNAs in the extracted RNA were detectable by RT-PCR. The blots can be stored at 4 degC for more than a few days to detect viral RNA, but not cellular RNA.
اظهر المزيد [+] اقل [-]