Occurrence of Meloidogyne microcephala in Oita Prefecture
2019
Suzuki, T. (Oita Prefectual Agriculture, forestry and Fisheries Research Center, Bungoohno, Oita (Japan)) | Yamano, H. | Iwahori, H.
Root-knot nematodes (RKN) are one of the most economically damaging nematodes in Japan as well as in the world. In November 2014, we found malformed taro (Colocasia esculenta) tubers in the field. Many RKN second juveniles were extracted from soil by the Baermann funnel method and female adults were found parasitizing on the tubers. Nematode species was identified with PCR-RFLP method using individual nematodes, and all RKN specimens were identified as Meloidogyne microcephala (Mm). We also identified RKN from the soil of several fields where taro’s seeds cultivated in the previous year (Field 2), tomato greenhouse and green pepper greenhouses neighboring to Field 2 (Field 3 and 4, respectively) and tomato greenhouse about 300 m apart from Field 2 (Field 5). The number of RKN (Meloidogyne incognita (Mi) / Mm) of Field 2, 3, 4 and 5 were 18/11, 15/23, 30/0 and 8/0, respectively.
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