High-yield degumming methods for preparing sericin from cocoons of mulberry and non-mulberry silkworms
2019
Karimazawa, M. (Biococoon Laboratories Inc., Research and Development Center, in collaboration with Morioka City and Iwate University, Morioka (Japan)) | Urita, S. | Yamashita, T. | Suzuki, K.
We developed high-yield degumming methods for preparing sericin from the cocoons of the mulberry silkworm Bombyx mori and the non-mulberry silkworms of Antheraea yamamai, and Cricula trifenestrata. We achieved sericin yields of 54%-55% from B. mori cocoons extracted in 0.24% NaOH, from A. yamamai cocoons extracted in 0.025% NaOH + 0.475% Na2CO3, and from C. trifenestrata cocoons extracted in 0.5% Na2CO3. In all three extracts, analysis of amino acid compositions indicated a high degree of sericin purity and Tricine-SDS-PAGE analysis indicated degradation to heterogeneous polypeptides. Thus, sericin preparations produced from the cocoons of silkworms by high-yield degumming methods offer promise for biomedical and industrial applications.
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