Neutrophil granules of the inshore hagfish Eptatretus burgeri infested with parasites
2020
Kondo, M. (National Fisheries University (Japan). Department of Applied Aquabiology) | Yasumoto, S.
Four kinds of parasites, Ceratomyxa sp. (Myxozoa), Myxinidocotyle japonica (Monogenea; Mj), nematodes (Nematode; species unidentified) and Acanthochondria eptatreti (Copepoda; Ae), were observed in inshore hagfish Eptatretus burgeri. Here, we report the morphological and cytochemical characteristics of neutrophils from inshore hagfish infested with each parasite. In all infested fish, two types of stratified granules with two-layer structure [inner layer (L0) and outer layer (L1)] were observed in the neutrophils [type 1, G-1 (chromophobic L0 and basophilic L1); type 2, G-2 (basophilic L0 and chromophobic L1)]. The L0 of G-2 from all parasite-infested fish show positive reaction to acid phosphatase and toluidine blue. Except for Ae-infested fish, L0 of G-2 was also Sudan black B positive. Beta-glucuronidase was detected in the L0 of G-2 of nematodes-infested fish. The neutrophils from Mj-infested fish and nematodes-infested fish were naphthol AS-D chloroacetate esterase positive, but show different positive sites: Mj-infested fish, L0 of G-1; nematodes-infested fish, L0 of G-2.
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