The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike
2024
Liping Shen | Lili Zhang | Changbin Yin | Xiaowan Xu | Yangyang Liu | Kuocheng Shen | He Wu | Zhiwen Sun | Ke Wang | Zhonghu He | Xueyong Zhang | Chenyang Hao | Jian Hou | Aoyue Bi | Xuebo Zhao | Daxing Xu | Botao Ye | Xuchang Yu | Ziying Wang | Danni Liu | Yuanfeng Hao | Fei Lu | Zifeng Guo
Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight (TGW) in wheat (Triticum aestivum L.). However, no mutations have been identified within the gene to test this association. The effects of TaSus1 on grain number per spike (GNS) also are largely unknown. Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike (FSN). In the present study, we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing. The triple mutants displayed lower FSN, GNS, grain number per spikelet (GNST), and TGW than wild-type plants. In 306 hexaploid wheat accessions, two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS. Introgression of the two alleles into a wheat genetic background confirmed their effects. The alleles differed in geographical distribution among the accessions.
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