A framework for assessing microbial degradation of organophosphate ester plasticizers in seawater

The assessment of persistence of organic pollutants in seawater is limited by the lack of user-friendly, quick protocols for assessing one of their main sinks, degradation by marine bacteria. Here we present an experimental workflow to identify organic pollutants degradation, taking organophosphate esters flame retardants and plasticizers (OPEs-FR-PL), as a model family of synthetic chemicals released into the marine environment that are particularly widespread due to their persistence and semi-volatile nature. The proposed novel workflow combines culture-dependent techniques, solvent demulsification-dispersive liquid-liquid microextraction, with quantitative liquid chromatography coupled with mass spectrometry analyses in order to identify marine bacterial isolates with the potential to degrade OPEs-FR-PL in the marine environment. This methodology evaluates growth rates, degradation capacities of different OPEs-FR-PL, and the ability of bacteria to utilize these pollutants as a sole source of carbon, phosphorus and energy. The proposed framework is more cost-effective than previous approaches as it is less time-consuming, reduces the use of solvents making it environmentally friendly, and can be used as a high throughput screening methodology. Although optimized here for OPEs-FR-PL degradation, this methodology can be adapted to a wide variety of contaminants of emerging concern. Using this developed workflow, we could detect that coastal Antarctic seawater harbors several bacterial taxa with the potential to degrade OPEs-FR-PL.

This work was supported by the Spanish Ministry of Science and Innovation through projects MIQAS (PID2021-128084OB-I00) and PANTOC (PID2021-127769NB-I00). I.S.-S. had the support of a Margarita Salas fellowship from the Universitat Autònoma de Barcelona (717638). The research group of Global Change and Genomic Biogeochemistry receives support from the Catalan Government (2021 SGR 00448). The authors wish to thank Dra R. Chaler and A. Garcia for their technical support during HPLC analysis.

Peer reviewed