Identification of Antibiotic Resistance Gene Hosts in Treatment Wetlands Using a Single-Cell Based High-Throughput Approach | Identification des gènes hôtes de la résistance aux antibiotiques dans les zones humides de traitement à l'aide d'une approche à haut débit basée sur une seule cellule
2023
Knecht, Camila, A | Hinkel, Maja | Mäusezahl, Ines | Kaster, Anne-Kristin | Nivala, Jaime | Müller, Jochen, A | Helmholtz Zentrum für Umweltforschung = Helmholtz Centre for Environmental Research (UFZ) | Karlsruhe Institute of Technology = Karlsruher Institut für Technologie (KIT) | Réduire, valoriser, réutiliser les ressources des eaux résiduaires (UR REVERSAAL) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | -The European Commission -the German Federal Ministry of Education and Research (BMBF; Grant Number 02WAP1616) within the framework of the collaborative international consortium NATURE, financed under the 2020 AquaticPollutantsJoint call of the AquaticPollutants ERA-NET Cofund (GA NO 869178). ERA-NET is an integral partof the activities developed by the Water, Oceans and AMR JPIs. - the Helmholtz Association of German Research Centers through its research program “PoF IV”- a doctoral stipend of the Henrich-Böll-Foundation
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Determining the prevalence of antimicrobial resistance (AMR) in non-clinical settings is vital for better management of the global AMR crisis. Untreated and even treated wastewaters are important sources that release AMR into the environment. Methodologically, it is difficult to generate a comprehensive in situ profile of antibiotic resistance gene hosts. Here, we used epicPCR (emulsion, paired isolation, and concatenation PCR) as a cultivation-independent method to reveal the host profiles of the AMR indicator genes intI1, sul1, sul2, and dfrA1 in two constructed wetlands treating municipal wastewater. Overall, the epicPCR analysis revealed a profile of AMR indicator gene hosts that is consistent with literature data from cultivation-based approaches. Most carriers of antibiotic resistance (AR) genes and likely of class 1 integrons belonged to the Gammaproteobateria, particularly the Burkholderiaceae and Rhodocyclaceae families, followed by members of the Campylobacterota, Desulfobacterota, and Firmicutes. The analysis also identified several novel hosts for the indicator genes widely distributed in the wetlands, including the genera Legionella and Ralstonia. Therefore, the application of epicPCR has produced an expanded insight into the in situ indicator gene host profile, while highlighting the role of the environment as a reservoir for AMR.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique