Epidermal UVA screening capacity measured in situ as an indicator of light acclimation state of leaves of a very plastic alpine plant Soldanella alpina L.
2020
Bidel, Luc | Meyer, Sylvie | Talhouët, Anne-Claire | Baudin, Xavier | Daniel, Caroline | Cazals, Guillaume | Streb, Peter | Diversité, Adaptation et Amélioration de la Vigne [AGAP] (DAAV) ; Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) | Ecologie Systématique et Evolution (ESE) ; Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS) | Université Paris Diderot, Sorbonne Paris Cité, Paris, France ; Université Paris Diderot - Paris 7 (UPD7) | Institut Jacques Monod (IJM (UMR_7592)) ; Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité) | Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM) ; Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) | Ecologie Systématique et Evolution (ESE) ; AgroParisTech-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Soldanella alpina differing in leaf epidermal UV-A absorbance (DEA(375)), as measured with the Dualex, was investigated as a model alpine plant for the flavonoid (Flay) composition and concentration and for anatomical and pigment characteristics. In sun leaves, twenty-three flavones were characterised by their mass formula, their maximum absorption, their glycosylation, their methylation and dehydroxylation pattern. The flavones belonged to four subfamilies (tetra-hydroxy-flavones, penta-hydroxy-flavones, penta-hydroxy-methyl-flavones and trihydroxy-di-methoxy-flavones), abundant in sun and shade leaves. Their concentration was estimated by their absorption at 350 nm after HPLC separation. Sun leaves contained relatively higher contents of penta-hydroxymethyl-flavones and shade leaves higher contents of tetra-hydroxy-flavones. The flavones were present mainly in vacuoles, all over the leaf. After shade-sun transfer, the content of most flavones increased, irrespective of the presence or absence of UV radiation. Highly significant correlations with the log-transformed DEA(375) suggest that DEA(375) can be readily applied to predict the flavone content of S. alpina leaves. Shade-sun transfer of leaves decreased the hydroxycinnamic acid (HCA) content, the mass-based chlorophyll (Chl) a + b content and the Chl/Carotenoid (Car) ratio but increased DEA(375), and the Car content. Together with previously reported anatomical characteristics all these parameters correlated significantly with the DEA(375). The Flay content is therefore correlated to most of the structural characteristics of leaf acclimation to light and this can be probed in situ by DEA(375).
اظهر المزيد [+] اقل [-]المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique
