First evidence of circulation of multiple arboviruses in Algeria
2024
Boubidi, Saïd | Mousson, Laurence | Kernif, Tahar | Khardine, Fayez | Hachid, Aïssam | Beck, Cécile | Lecollinet, Sylvie | Moraes, Rayane | Moutailler, Sara | Dauga, Catherine | Failloux, Anna-Bella | Institut Pasteur d'Algérie ; Pasteur Network (Réseau International des Instituts Pasteur) | Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors ; Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité) | Université d'Alger 1 (Benyoucef Benkhedda) | Virologie UMR1161 (VIRO) ; École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR) ; École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | This work was supported by Pasteur Institute of Algeria in the frame of the MATI Program of the Institut Pasteur International Network “ENVIRONmental changes and MOSquito-borne diseases: the example of West Nile (Environ-MOS)”. It was also partially supported by grants from The French Agency for Food, Environmental and Occupational Health (ANSES).
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Algeria like other North African countries is experiencing recurrent episodes of West Nile Virus (WNV) emergences and new health threats associated with the introduction of Aedes albopictus in 2010 are to be feared. To improve the surveillance of mosquito-borne pathogens, we performed a study using innovative tools based on multiplex molecular methods. Methods We combined two approaches: a high-throughput chip based on the BioMark Dynamic array system to detect arboviruses in mosquitoes, and a set of immunologic methods (ELISA, microsphere immunoassays (MIA) and virus microneutralization tests (MNT)) for serological surveys in animal hosts. We investigated two distinct regions: a first zone located in the coastal humid region and a second one in the Saharan desert region. Principal findings We collected a total of 1,658 mosquitoes belonging to nine different species and found predominantly Culex pipienss . l . (56.5%) and Cx . perexiguus (27.5%). From 180 pools of 10 mosquitoes, we detected four arboviruses: Banna virus (BAV), chikungunya virus (CHIKV), Sindbis virus (SINV), and Usutu virus (USUV). Moreover, we examined 389 blood samples from equids and poultry and found that 52.4% were positive for flavivirus antibodies in ELISA, while 30.8% were positive for WNV and two chickens and two equids were positive for USUV by MNT and MIA respectively. Conclusions To our knowledge, this is the first report of five arboviruses circulating in Algeria, with three reported for the first time (CHIKV, BAV, and USUV). Our study brings evidence that reinforcing surveillance using more discriminant tools may help in anticipating future emergences and propose adapted control measures.
اظهر المزيد [+] اقل [-]المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique