OsWRKY22, a monocot WRKY gene, plays a role in the resistance response to blast
2012
Abbruscato, Pamela | Nepusz, Tamas | Mizzi, Luca | del Corvo, Marcello | Morandini, Piero | Fumasoni, Irene | Michel, Corinne | Paccanaro, Alberto | Guiderdoni, Emmanuel | Schaffrath, Ulrich | Morel, Jean-Benoit, J.-B. | Piffanelli, Pietro | Faivre-Rampant, Odile | Parco Tecnologico Padano | University of London | Università degli Studi di Milano = University of Milan (UNIMI) | Istituto di Biofisica | Institut National de la Recherche Agronomique (INRA) | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | RWTH Aachen University = Rheinisch-Westfälische Technische Hochschule Aachen (RWTH Aachen) | Biologie et Génétique des interactions Plantes-parasites pour la Protection Intégrée ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Cariplo Foundation (Italy) [2005-1866, 2008-3179]; Newton Fellowship Scheme of the Royal Society [NF080750]; Biotechnology and Biological Sciences Research Council (BBSRC) [BB/F00964X/1]; Agropolis Foundation
BGPI : équipe 4
اظهر المزيد [+] اقل [-]International audience
اظهر المزيد [+] اقل [-]إنجليزي. With the aim of identifying novel regulators of host and nonhost resistance to fungi in rice, we carried out a systematic mutant screen of mutagenized lines. Two mutant wrky22 knockout lines revealed clear-cut enhanced susceptibility to both virulent and avirulent Magnaporthe oryzae strains and altered cellular responses to nonhost Magnaporthe grisea and Blumeria graminis fungi. In addition, the analysis of the pathogen responses of 24 overexpressor OsWRKY22 lines revealed enhanced resistance phenotypes on infection with virulent M. oryzae strain, confirming that OsWRKY22 is involved in rice resistance to blast. Bioinformatic analyses determined that the OsWRKY22 gene belongs to a well-defined cluster of monocot-specific WRKYs. The co-regulatory analysis revealed no significant co-regulation of OsWRKY22 with a representative panel of OsWRKYs, supporting its unique role in a series of transcriptional responses. In contrast, inquiring a subset of biotic stress-related Affymetrix data, a large number of resistance and defence-related genes were found to be putatively co-expressed with OsWRKY22. Taken together, all gathered experimental evidence places the monocot-specific OsWRKY22 gene at the convergence point of signal transduction circuits in response to both host and nonhost fungi encountering rice plants.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique