Setae from the pine processionary moth (Thaumetopoea pityocampa) contain several relevant allergens
2012
Rodríguez-Mahillo, Ana Isabel | González-Muñoz, Miguel | Vega, José María | López, Juan Antonio | Yart, Annie | Kerdelhue, Carole | Camafeita, Emilio | Ortiz, José Carlos Garcia | Vogel, Heiko | Toffolo, Edoardo Petrucco | Zovi, Daniel | Battisti, Andrea | Roques, Alain | Moneo, Ignacio | Instituto de Salud Carlos III [Madrid] (ISCIII) | Rio Hortega University Hospital (Hospital Universitario Río Hortega) [Valladolid, Spain] (RHUH) | Unidad de Proteomica ; Centro de Investigacion Medica Aplicada (CIMA) | Unité de recherche Zoologie Forestière (URZF) ; Institut National de la Recherche Agronomique (INRA) | Centre de Biologie pour la Gestion des Populations (UMR CBGP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [Occitanie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Department of Entomology ; Michigan State University [East Lansing] ; Michigan State University System-Michigan State University System | Università degli Studi di Padova = University of Padua (Unipd) | URTICLIM; Agence Nationale de la Recherche [ANR 07BDIV 013]; Instituto de Salud Carlos III, Ministerio de Ciencia e Innovacion, Spain [CA07/00046]
International audience
اظهر المزيد [+] اقل [-]إنجليزي. Background. Pine processionary larvae produce urticating hairs (setae) that serve for protection against predators. Setae induce cutaneous reactions in animals and humans. The presence of toxic or allergic mechanisms is a matter of debate. Objectives. To detect the presence of allergens in setae and to characterize them. Materials and methods. Setae extracts were characterized by gel staining and immunoblot, with sera from patients with immediate reactions and positive prick test reactions, as well as a rabbit antiserum raised against setae. Setae proteins were fractionated by high-performance liquid chromatography. The most relevant allergen was analysed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), and its sequence was deduced from an expressed sequence tag bank. Results. Setae contained at least seven different allergens. The most intense detection corresponded to a protein of MW ∼14 000 that was similar to thaumetopoein, a previously described protein with mast cell-degranulating properties. MALDI-MS-based de novo sequencing provided a partial amino acid sequence different from that of the previously described allergen Tha p 1, and it was named Tha p 2. This allergen was detected in 61% of patients, and it is therefore a new major caterpillar allergen. Conclusions. Penetration of the setae from the pine processionary caterpillar delivers their allergenic content in addition to causing mechanical or toxic injury
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique