Systems approaches provide new insights into Arabidopsis thaliana root growth under mineral nutrient limitation
2018
Bouain, Nadia | Korte, Arthur | Satbhai, Santosh B. | Rhee, Seung Y. | Busch, Wolfgang | Rouached, Hatem | Biochimie et Physiologie Moléculaire des Plantes (BPMP) ; Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Evolutionary Genomics Center for Computational and Theoretical Biology (CCTB) | Julius-Maximilians-Universität Würzburg = University of Würzburg [Würsburg, Germany] (JMU) | Austrian Academy of Sciences, Vienna Biocenter ; Gregor Mendel Institute | Integrative Biology Laboratory, Salk Institute for Biological Studies ; Plant Molecular and Cellular Biology Laboratory | Department of Plant Biology [Carnegie] (DPB) ; Carnegie Institution for Science
preprint déposé dans bioRxiv. 2018
اظهر المزيد [+] اقل [-]إنجليزي. The molecular genetic mechanisms by which plants modulate their root growth rate (RGR) in response to nutrient deficiency are largely unknown. Using a panel of Arabidopsis thaliana natural accessions, we provide a comprehensive combinatorial analysis of RGR variation under macro- and micronutrient deficiency, namely phosphorus (P), iron (Fe), and zinc (Zn), which affect root growth in opposite directions. We found that while -P stimulates early RGR of most accessions, -Fe or -Zn reduces it. The combination of either -P-Fe or -P-Zn leads to suppression of the growth inhibition exerted by -Fe or -Zn alone. Surprisingly, Arabidopsis reference accession Columbia (Col-0) is not representative of the species under -P and -Zn. Using a genome wide association study, we identify candidate genes that control RGR under the assayed nutrient deficiency conditions. By using a network biology driven search using these candidate genes, we further identify a functional module enriched in regulation of cell cycle, DNA replication and chromatin modification that possibly underlies the suppression of root growth reduction in -P-Fe conditions. Collectively, our findings provide a framework for understanding the regulation of RGR under nutrient deficiency, and open new routes for the identification of both large effect genes and favorable allelic variations to improve root growth.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل Institut national de la recherche agronomique