Generation of rabbit pluripotent stem cell lines
2012
Dinnyes, A., A. | Pirity, M.K., M. | Gocza, E., E. | Osteil, P., P. | Daniel, Nathalie, N. | Tancos, Z., Z. | Polgar, Z., Z. | Maraghechi, P., P. | Ujhelly, O., O. | Nemes, C., C. | Stout, T., T. | Tapponnier, Yann, Y. | Bosze, Z., Z. | Jouneau, Alice | Afanassieff, Marielle | Savatier, P., P. | Molecular Animal Biotechnology Laboratory ; Szent István University | Faculty of Veterinary, Department of Farm Animal Health ; Universiteit Utrecht / Utrecht University [Utrecht] | Institut cellule souche et cerveau (SBRI) ; Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM) | Biologie du développement et reproduction (BDR) ; École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) | Faculty of Veterinary Medicine, Department of Equine Health ; Universiteit Utrecht / Utrecht University [Utrecht] | EU FP7 (PartnErS, PIAP-GA-2008-218205; induHeart, PEOPLE-IRG-2008-234390; InduWr, PEOPLE-IRG-2009-245808; RabPstem, PERGOT-GA-2010-268422; PlurisYs, HEALTH-2007-B-223485; AniStem, PIAP-GA-2011-288264), NKTH-OTKA-EU-7KP-HUMAN-MB08-C-80-205; Plurabbit, OMFB-00130-00131/2010 ANR-NKTH/09-GENM-010-01 | International Embryo Transfer Society (IETS). USA.
Pluripotent stem cells have the capacity to divide indefinitely and to differentiate to all the somatic tissues. They can be genetically manipulated in vitro by knocking in and out genes, therefore they serve as an excellent tool for gene-function studies and for the generation of models for human diseases. Since 1981, when the first mouse embryonic stem cell (ESC) line was generated, several attempts have been made to generate pluripotent stem cells from other species as it would help us to understand the differences and similarities of signaling pathways involved in pluripotency and differentiation, and would reveal whether the fundamental mechanism controlling self-renewal of pluripotent cells is conserved among different species. This review gives an overlook of embryonic and induced pluripotent stem cell (iPSCs) research in the rabbit which is one of the most relevant non-rodent species for animal models. To date, several lines of putative ESCs and iPSCs have been described in the rabbit. All expressed stem cell-associated markers and exhibited longevity and pluripotency in vitro, but none have been proven to exhibit full pluripotency in vivo. Moreover, similarly to several domestic species, markers used to characterize the putative ESCs are not fully adequate because studies in domestic species have revealed that they are not specific to the pluripotent inner cell mass. Future validation of rabbit pluripotent stem cells would benefit greatly from a reliable panel of molecular markers specific to pluripotent cells of the developing rabbit embryo. The status of isolation and characterization of the putative pluripotency genes in rabbit will be discussed. Using rabbit specific pluripotency genes we might be able to reprogram somatic cells and generate induced pluripotent stem cells more efficiently thus overcome some of the challenges towards hamessing the potential of this technology.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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تم تزويد هذا السجل من قبل Institut national de la recherche agronomique