miR-210 promotes immune- and suppresses oocyte meiosis-related genes in the zebrafish ovarian cells

12 pages, 13 figures, 2 tables, supplementary material https://doi.org/10.1016/j.ygeno.2024.110820.-- Data availability: Data will be made available on request

microRNA-210 (miRNA), a well-documented miRNA, has been implicated in a myriad of biological processes, including responses to hypoxia, angiogenesis, cell proliferation, and male infertility in humans. However, a comprehensive understanding of its functions in fish requires further investigation. This study pursued to elucidate the downstream effect of dre-miR-210-5p on primary ovarian cell culture in zebrafish (Danio rerio), an animal model. A protocol was settled down by incubations with either an miR-210 mimic or a scrambled miRNA in the isolated ovaries. RNA-sequencing analysis identified ∼6000 differentially expressed target genes revealing that downregulated genes were associated with reproduction-related pathways while immune-related pathways displayed an upregulated pattern. To identify molecular markers, predicted target genes were classified into reproduction and immune cell types. These findings underscore the existence of a profound interplay between the reproductive and immune systems, with miR-210 emerging as a pivotal player in orchestrating transcriptomic alterations within fish ovaries

This study was supported by the Spanish Ministry of Science and Innovation grant 2PID2020-113781RB-I00 “MicroMet” and by the Consejo Superior de Investigaciones Científicas (CSIC) grant 02030E004 “Interomics” to LR. [...] We thank the support by funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence accreditation (CEX2019–000928-S) to ICM

Peer reviewed