Thriving beneath olive trees: The influence of organic farming on microbial communities

Soil health and root-associated microbiome are interconnected factors involved in plant health. The use of manure amendment on agricultural fields exerts a direct benefit on soil nutrient content and water retention, among others. However, little is known about the impact of manure amendment on the root-associated microbiome, particularly in woody species. In this study, we aimed to evaluate the effects of ovine manure on the microbial communities of the olive rhizosphere and root endosphere. Two adjacent orchards subjected to conventional (CM) and organic (OM) management were selected. We used metabarcoding sequencing to assess the bacterial and fungal communities. Our results point out a clear effect of manure amendment on the microbial community. Fungal richness and diversity were increased in the rhizosphere. The fungal biomass in the rhizosphere was more than doubled, ranging from 1.72 × 10 ± 1.62 × 10 (CM) to 4.54 × 10 ± 8.07 × 10 (OM) copies of the 18 S rRNA gene g soil. Soil nutrient content was also enhanced in the OM orchard. Specifically, oxidable organic matter, total nitrogen, nitrate, phosphorous, potassium and sulfate concentrations were significantly increased in the OM orchard. Moreover, we predicted a higher abundance of bacteria in OM with metabolic functions involved in pollutant degradation and defence against pathogens. Lastly, microbial co-occurrence network showed more positive interactions, complexity and shorter geodesic distance in the OM orchard. According to our results, manure amendment on olive orchards represents a promising tool for positively modulating the microbial community in direct contact with the plant.

This research was funded by grants AGL2016-75729-C2-1-R from the Spanish Ministerio de Economía y Competitividad and PID2019-106283RB-I00 from Spanish Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación, both co-financed by the European Regional Development Fund (ERDF). These funding sources had no involvement in this work preparation.

Peer reviewed