A consortium of different Saccharomyces species enhances the content of bioactive tryptophan-derived compounds in wine fermentations

In recent years, the presence of molecules derived from aromatic amino acids in wines has been increasingly demonstrated to have a significant influence on wine quality and stability. In addition, interactions between different yeast species have been observed to influence these final properties. In this study, a screening of 81 yeast strains from different environments was carried out to establish a consortium that would promote the improvement of indolic compound levels in wine. Two strains, Saccharomyces uvarum and Saccharomyces eubayanus, with robust fermentative capacity were selected to be combined with a Saccharomyces cerevisiae strain with a predisposition towards the production of indolic compounds. Fermentation dynamics were studied in pure cultures, co-inoculations and sequential inoculations, analysing strain interactions and end-of-fermentation characteristics. Fermentations showing significant interactions were further analyzed for the resulting indolic compounds and aroma profile, with the aim of observing potential interactions and synergies resulting from the combination of different strains in the final wine. Sequential inoculation of S. cerevisiae after S. uvarum or S. eubayanus was observed to increase indolic compound levels, particularly serotonin and 3-indoleacetic acid. This study is the first to demonstrate how the formation of microbial consortia can serve as a useful strategy to enhance compounds with interesting properties in wine, paving the way for future studies and combinations.

This research was funded by MCIN/AEI/10.13039/501100011033, grant numbers PID2019-108722RB-C31 and PID2019-108722RB-C32, and by Generalitat Valenciana, grant number PROMETEO/2020/014. APC thank to FPU19/02060 grant funded by “ESF Investing in your future” (MCIN/AEI/10.13039/501100011033). Finally, JMG acknowledges the award of the Spanish government MCIN/AEI to the IATA-CSIC as Center of Excellence (CEX2021-001189-S/ MICIU/AEI / 10.13039/501100011033).

With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX 2021-001189-S)

Peer reviewed